Overview

  • Product nameAnti-Kinetochore antibody
    See all Kinetochore primary antibodies
  • Description
    Rabbit polyclonal to Kinetochore
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, corresponding to a region within residues 375 and 425 of human Kinetochore (NP_055523.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • HsROD antibody
      • Kinetochore associated 1 antibody
      • KNTC1 antibody
      • ROD antibody
      • Rough Deal homolog, centromere/kinetochore protein antibody
      see all

    Anti-Kinetochore antibody images

    • All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 250 kDa
      Observed band size : 250 kDa
      Additional bands at : 115 kDa,175 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds
    • Detection of Kinetochore by Western Blot of Immunprecipitate.
      ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    References for Anti-Kinetochore antibody (ab85996)

    ab85996 has not yet been referenced specifically in any publications.

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