Anti-Kinetochore antibody (ab85996)
Key features and details
- Rabbit polyclonal to Kinetochore
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kinetochore antibody
See all Kinetochore primary antibodies -
Description
Rabbit polyclonal to Kinetochore -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis, Platypus -
Immunogen
Synthetic peptide corresponding to Human Kinetochore aa 350-450.
Database link: P50748 -
Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85996 was affinity purified using an epitope specific to Kinetochore immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 250 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 9735 Human
- Omim: 607363 Human
- SwissProt: P50748 Human
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Alternative names
- HsROD antibody
- Kinetochore associated 1 antibody
- KNTC1 antibody
see all
Images
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All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 115 kDa, 175 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Detection of Kinetochore by Western Blot of Immunprecipitate.
ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab85996 has been referenced in 1 publication.
- Liu CT et al. shRNA-mediated knockdown of KNTC1 suppresses cell viability and induces apoptosis in esophageal squamous cell carcinoma. Int J Oncol 54:1053-1060 (2019). PubMed: 30628654