Overview

  • Product nameAnti-Kinetochore antibody
    See all Kinetochore primary antibodies
  • Description
    Rabbit polyclonal to Kinetochore
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MWNDIELLTN DDTGSGYLSV GSRKEHGTAL YQVDLLVKIS SEKASLNPKI) of Human Kinetochore (NP_055523)

  • Positive control
    • OVCAR3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab97841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 251 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • HsROD antibody
    • Kinetochore associated 1 antibody
    • KNTC1 antibody
    • ROD antibody
    • Rough Deal homolog, centromere/kinetochore protein antibody
    see all

Anti-Kinetochore antibody images

  • Anti-Kinetochore antibody (ab97841) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 251 kDa

References for Anti-Kinetochore antibody (ab97841)

ab97841 has not yet been referenced specifically in any publications.

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