Overview

  • Product nameAnti-Kininogen 1 antibody [EPR6097]
    See all Kininogen 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6097] to Kininogen 1
  • SpecificityThis antibody detects three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Kininogen 1.

  • Positive control
    • Human plasma, platelet and ovarian cancer lysates; Human kidney tissue, Human ovarian adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 2.70 x 10 -12 M Learn more about KD
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6097
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab124737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 45, 56, 120 kDa (predicted molecular weight: 48, 72 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min

Target

  • Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similaritiesContains 3 cystatin domains.
  • Post-translational
    modifications
    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-2-thiol proteinase inhibitor antibody
    • BDK antibody
    • BK antibody
    • Bradykinin antibody
    • Fitzgerald factor antibody
    • High molecular weight kininogen antibody
    • HMWK antibody
    • Ile-Ser-Bradykinin antibody
    • Kallidin I antibody
    • Kallidin II antibody
    • Kininogen antibody
    • KNG antibody
    • KNG1 antibody
    • KNG1_HUMAN antibody
    • Low molecular weight growth-promoting factor antibody
    • Williams-Fitzgerald-Flaujeac factor antibody
    see all

Anti-Kininogen 1 antibody [EPR6097] images

  • ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human kidney tissues by Immunohistochemistry.
  • ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • All lanes : Anti-Kininogen 1 antibody [EPR6097] (ab124737) at 1/10000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human platelet lysate
    Lane 3 : Human ovarian cancer lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 48, 72 kDa
    ab124737 is detecting three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).

References for Anti-Kininogen 1 antibody [EPR6097] (ab124737)

This product has been referenced in:
  • Schrödter S  et al. Identification of the dopamine transporter SLC6A3 as a biomarker for patients with renal cell carcinoma. Mol Cancer 15:10 (2016). WB ; Human . Read more (PubMed: 26831905) »
  • Yao Y  et al. Screening and identification of potential predictive biomarkers for Down's syndrome from second trimester maternal serum. Expert Rev Proteomics 12:97-107 (2015). WB ; Human . Read more (PubMed: 25434962) »

See all 2 Publications for this product

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