• Product nameAnti-Kir2.1 antibody
    See all Kir2.1 primary antibodies
  • Description
    Rabbit polyclonal to Kir2.1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the N terminal domain of human Kir2.1.



Our Abpromise guarantee covers the use of ab65796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 48 kDa.
IHC-P 1/1000.
ICC/IF 1/1000.


  • FunctionProbably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
  • Tissue specificityHeart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
  • Involvement in diseaseDefects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
  • Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac inward rectifier potassium channel antibody
    • HHBIRK 1 antibody
    • HHBIRK1 antibody
    • HHIRK 1 antibody
    • HHIRK1 antibody
    • HIRK 1 antibody
    • hIRK1 antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.1 antibody
    • Inward rectifier potassium channel 2 antibody
    • inwardly rectifying subfamily J member 2 antibody
    • IRK 1 antibody
    • IRK-1 antibody
    • IRK1 antibody
    • IRK2_HUMAN antibody
    • KCNJ2 antibody
    • KIR2.1 antibody
    • LQT 7 antibody
    • LQT7 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 2 antibody
    • Potassium inwardly rectifying channel J2 antibody
    • Potassium inwardly rectifying channel subfamily J member 2 antibody
    • SQT 3 antibody
    • SQT3 antibody
    see all

Anti-Kir2.1 antibody images

  • ICC/IF image of ab65796 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65796, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab65796 staining Kir2.1 in human colon.
    Left panel: with primary antibody at 1:1000 dilution. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Kir2.1 antibody (ab65796)

This product has been referenced in:
  • Geraldes V  et al. Chronic depression of hypothalamic paraventricular neuronal activity produces sustained hypotension in hypertensive rats. Exp Physiol 99:89-100 (2014). Rat . Read more (PubMed: 24142454) »
  • Geraldes V  et al. Essential role of RVL medullary neuronal activity in the long term maintenance of hypertension in conscious SHR. Auton Neurosci 186:22-31 (2014). Read more (PubMed: 25283065) »

See all 3 Publications for this product

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