Recombinant
RabMAb

Anti-Kir2.1 antibody [EPR4530] (ab109750)

Overview

  • Product name
    Anti-Kir2.1 antibody [EPR4530]
    See all Kir2.1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4530] to Kir2.1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human Kir2.1.

  • Positive control
    • 293T and A549 cell lysates; Human brain tissue; SH SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4530
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
    • Tissue specificity
      Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
    • Involvement in disease
      Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
      Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
    • Sequence similarities
      Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac inward rectifier potassium channel antibody
      • HHBIRK 1 antibody
      • HHBIRK1 antibody
      • HHIRK 1 antibody
      • HHIRK1 antibody
      • HIRK 1 antibody
      • hIRK1 antibody
      • Inward rectifier K antibody
      • Inward rectifier K(+) channel Kir2.1 antibody
      • Inward rectifier potassium channel 2 antibody
      • inwardly rectifying subfamily J member 2 antibody
      • IRK 1 antibody
      • IRK-1 antibody
      • IRK1 antibody
      • IRK2_HUMAN antibody
      • KCNJ2 antibody
      • KIR2.1 antibody
      • LQT 7 antibody
      • LQT7 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 2 antibody
      • Potassium inwardly rectifying channel J2 antibody
      • Potassium inwardly rectifying channel subfamily J member 2 antibody
      • SQT 3 antibody
      • SQT3 antibody
      see all

    Images

    • All lanes : Anti-Kir2.1 antibody [EPR4530] (ab109750) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa
    • ab109750 at 1/250 dilution staining Kir2.1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109750 at 1/100 dilution staining Kir2.1 in SH SY5Y cells by Immunofluorescence.

    References

    ab109750 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your message.

    I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.

    I would like to reassure you that ab65796 and ab80969 have bee...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted May 10 2013

    Thank youand your customer very much for your interest in our antibodies.

    I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

    Only one antibody sho...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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