• Product nameAnti-Kir2.1 antibody [S21-32]
    See all Kir2.1 primary antibodies
  • Description
    Mouse monoclonal [S21-32] to Kir2.1
  • SpecificityNo cross reactivity against Kir2.2, or Kir2.3
  • Tested applicationsSuitable for: WB, ICC, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 41-64 and 189-428 of mouse Kir2.1 (NP_032451).

  • Positive control
    • Rat brain lysate. IF/ICC: SKNSH cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS21-32
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab85492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. If bands are weak, use lysate without boiling, heat at 37°C for 15 minutes.
ICC Use a concentration of 0.1 - 1 µg/ml. (Perox)
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionProbably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
  • Tissue specificityHeart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
  • Involvement in diseaseDefects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
  • Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac inward rectifier potassium channel antibody
    • HHBIRK 1 antibody
    • HHBIRK1 antibody
    • HHIRK 1 antibody
    • HHIRK1 antibody
    • HIRK 1 antibody
    • hIRK1 antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.1 antibody
    • Inward rectifier potassium channel 2 antibody
    • inwardly rectifying subfamily J member 2 antibody
    • IRK 1 antibody
    • IRK-1 antibody
    • IRK1 antibody
    • IRK2_HUMAN antibody
    • KCNJ2 antibody
    • KIR2.1 antibody
    • LQT 7 antibody
    • LQT7 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 2 antibody
    • Potassium inwardly rectifying channel J2 antibody
    • Potassium inwardly rectifying channel subfamily J member 2 antibody
    • SQT 3 antibody
    • SQT3 antibody
    see all

Anti-Kir2.1 antibody [S21-32] images

  • ICC/IF image of ab85492 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (abX85492, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab85492 staining Kir2.1 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Overlay histogram showing SH-SY5Y cells stained with ab85492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Kir2.1 antibody [S21-32] (ab85492)

This product has been referenced in:
  • Goldoni D  et al. A novel dual-fluorescence strategy for functionally validating microRNA targets in 3-prime untranslated regions: regulation of the inward rectifier potassium channel Kir2.1 by miR-212. Biochem J : (2012). WB ; Human . Read more (PubMed: 22880819) »

See 1 Publication for this product

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