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Fusion protein, corresponding to amino acids 41-64 and 189-428 of mouse Kir2.1 (NP_032451).
Our Abpromise guarantee covers the use of ab85492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. If bands are weak, use lysate without boiling, heat at 37°C for 15 minutes.|
|ICC||Use a concentration of 0.1 - 1 µg/ml. (Perox)|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ICC/IF image of ab85492 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (abX85492, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"