Overview

  • Product nameAnti-Kir2.2 antibody [S24-1]
    See all Kir2.2 primary antibodies
  • Description
    Mouse monoclonal [S24-1] to Kir2.2
  • SpecificityDoes not cross react with Kir2.1 and Kir2.3
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein: LVENKFLLPS ANSFCYENEL AFLSRDEEDE VATDRDGRSP QPEHDFDRLQ ASSGALERPY RRESEI, corresponding to amino acids 362-427 of Rat Kir2.2 (NP_446433).

  • Positive control
    • Rat brain lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS24-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProbably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
  • Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP sensitive inward rectifier potassium channel 12 antibody
    • ATP-sensitive inward rectifier potassium channel 12 antibody
    • hIRK antibody
    • Human high conductance inward rectifier potassium channel alpha antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.2 antibody
    • Inward rectifier K(+) channel Kir2.2v antibody
    • inwardly rectifying subfamily J member 12 antibody
    • IRK-2 antibody
    • IRK12_HUMAN antibody
    • IRK2 antibody
    • KCNJ12 antibody
    • kcnj12x antibody
    • KCNJN1 antibody
    • Kir2.2v antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 12 antibody
    • Potassium inwardly rectifying channel subfamily J member 12 antibody
    • Potassium inwardly rectifying channel,subfamily J inhibitor 1 antibody
    see all

Anti-Kir2.2 antibody [S24-1] images

  • All lanes : Anti-Kir2.2 antibody [S24-1] (ab84821)

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg


    Predicted band size : 48 kDa
  • ab84821 staining Kir2.2 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab84821 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84821, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Kir2.2 antibody [S24-1] (ab84821)

This product has been referenced in:
  • Sivagangabalan G  et al. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PLoS One 9:e82179 (2014). WB ; Human . Read more (PubMed: 24427266) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"