Overview

  • Product name
    Anti-KIR2.3 antibody [N25/35]
    See all KIR2.3 primary antibodies
  • Description
    Mouse monoclonal [N25/35] to KIR2.3
  • Host species
    Mouse
  • Specificity
    Does not cross react with Kir2.1 and Kir2.2
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein: AAAAAAVAAG LGLEAGSKEE AGIIRMLEFG SHLDLERMQA ATLPLDNISY RRESA, corresponding to amino acids 390-445 of Human KIR2.3 (NP_032453).

  • Positive control
    • Rat brain lysate.
  • General notes

    The clone number has been updated from S25-35 to N25/35, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N25/35
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 45 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
  • Tissue specificity
    Heart, skeletal muscle, and several different brain regions including the hippocampus.
  • Sequence similarities
    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.
  • Domain
    The Val/Gly/Ala/Pro stretch may have a functional role in the conductance or permeation properties.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle membrane. TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hippocampal inward rectifier antibody
    • Hippocampal inward rectifier potassium channel antibody
    • HIR antibody
    • HIRK2 antibody
    • HRK1 antibody
    • Inward rectifier K(+) channel Kir2.3 antibody
    • Inward rectifier K+ channel Kir2.3 antibody
    • Inward rectifier potassium channel 4 antibody
    • Inward rectifier potassium channel Kir 2.3 antibody
    • inwardly rectifying subfamily J member 4 antibody
    • IRK-3 antibody
    • IRK3 antibody
    • IRK4_HUMAN antibody
    • KCNJ 4 antibody
    • Kcnj4 antibody
    • Kir2.3 antibody
    • MGC142066 antibody
    • MGC142068 antibody
    • OTTHUMP00000028922 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 4 antibody
    • Potassium inwardly rectifying channel J4 antibody
    • Potassium inwardly rectifying channel subfamily J member 4 antibody
    see all

Images

  • All lanes : Anti-KIR2.3 antibody [N25/35] (ab84822) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg
  • Overlay histogram showing SH-SY5Y cells stained with ab84822 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84822, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Chen Y  et al. MicroRNAs 221/222 and genistein-mediated regulation of ARHI tumor suppressor gene in prostate cancer. Cancer Prev Res (Phila) 4:76-86 (2011). WB ; Human . Read more (PubMed: 21071579) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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