Overview

  • Product name
  • Description
    Mouse monoclonal to Kir4.1
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Zebrafish, Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 276-380 of Human KCNJ10

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab55380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.
    • Tissue specificity
      Expressed in kidney (at protein level).
    • Involvement in disease
      Seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance
    • Sequence similarities
      Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
    • Cellular localization
      Membrane. Basolateral cell membrane. In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.
    • Information by UniProt
    • Database links
    • Alternative names
      • inwardly rectifying subfamily J member 10 antibody
      • ATP dependent inwardly rectifying potassium channel Kir4.1 antibody
      • ATP sensitive inward rectifier potassium channel 10 antibody
      • ATP-dependent inwardly rectifying potassium channel Kir4.1 antibody
      • ATP-sensitive inward rectifier potassium channel 10 antibody
      • BIRK10 antibody
      • Glial ATP dependent inwardly rectifying potassium channel KIR4.1 antibody
      • Inward rectifier K(+) channel Kir1.2 antibody
      • Inward rectifier K+ channel KIR1.2 antibody
      • Inwardly rectifying potassium channel Kir1.2 antibody
      • KCJ10_HUMAN antibody
      • KCNJ 10 antibody
      • Kcnj10 antibody
      • KCNJ13 PEN antibody
      • KIR1.2 antibody
      • KIR4.1 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 10 antibody
      • Potassium inwardly rectifying channel subfamily J member 10 antibody
      • SESAME antibody
      see all

    Images

    • ab55380 staining Kir4.1 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS, for 60 minutes at 23°C, followed by staining with ab55380 at 1/200 in blocking solution for 16h at 4°C. An alexa 647conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Kir4.1 expression can be observed in the IPL (inner plexiform layer) in purple.

      See Abreview

    • Western blot against tagged recombinant protein immunogen using ab55380 KCNJ10 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References

    ab55380 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Sep 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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