Zebrafish, Recombinant fragment Predicted to work with:
Recombinant fragment, corresponding to amino acids 276-380 of Human KCNJ10
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.
Expressed in kidney (at protein level).
Involvement in disease
Seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
Membrane. Basolateral cell membrane. In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.
Potassium channel inwardly rectifying subfamily J member 10 antibody
Potassium inwardly rectifying channel subfamily J member 10 antibody
Immunohistochemistry (Frozen sections) - Anti-Kir4.1 antibody (ab55380)This image is courtesy of an Abreview submitted by Dr Ryan MacDonald
ab55380 staining Kir4.1 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS, for 60 minutes at 23°C, followed by staining with ab55380 at 1/200 in blocking solution for 16h at 4°C. An alexa 647conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Kir4.1 expression can be observed in the IPL (inner plexiform layer) in purple.