Anti-Kir6.2/BIR antibody (ab79171)
Key features and details
- Rabbit polyclonal to Kir6.2/BIR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Kir6.2/BIR antibody
See all Kir6.2/BIR primary antibodies -
Description
Rabbit polyclonal to Kir6.2/BIR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, PBS, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Relevance
ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus. -
Cellular localization
Cell Membrane; Multi-pass membrane protein -
Database links
- Entrez Gene: 3767 Human
- Entrez Gene: 16514 Mouse
- Omim: 600937 Human
- SwissProt: Q14654 Human
- SwissProt: Q61743 Mouse
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Alternative names
- ATP sensitive inward rectifier potassium channel 11 antibody
- Beta cell inward rectifier subunit antibody
- BIR antibody
see all
Images
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All lanes : Anti-Kir6.2/BIR antibody (ab79171) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated with heat shock
Lane 2 : extracts from NIH3T3 cells treated with heat shock
Lane 3 : extracts from NIH3T3 cells treated with heat shock with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunohistochemical analysis of Kir6.2/BIR in paraffin embedded human brain tissue using a 1/50 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
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Immunofluorescence analysis of Kir6.2/BIR in HeLa cells, using a 1/500 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab79171 has been referenced in 7 publications.
- Wei C et al. The reversible effects of free fatty acids on sulfonylurea-stimulated insulin secretion are related to the expression and dynamin-mediated endocytosis of KATP channels in pancreatic β cells. Endocr Connect 12:N/A (2023). PubMed: 36398885
- Jiang Z et al. Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes. EMBO J 41:e106825 (2022). PubMed: 35023164
- Bai XJ et al. Glucagon-Like Peptide-1 Analog Liraglutide Attenuates Pressure-Overload Induced Cardiac Hypertrophy and Apoptosis through Activating ATP Sensitive Potassium Channels. Cardiovasc Drugs Ther 35:87-101 (2021). PubMed: 33057968
- Okada T et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
- Hu D et al. Identification, clinical manifestation and structural mechanisms of mutations in AMPK associated cardiac glycogen storage disease. EBioMedicine 54:102723 (2020). PubMed: 32259713
- Gong XG et al. Da-bu-yin-wan and qian-zheng-san to neuroprotect the mouse model of Parkinson's disease. Evid Based Complement Alternat Med 2014:729195 (2014). WB . PubMed: 25610480
- Raeis-Dauvé V et al. Increased expression of adenosine triphosphate-sensitive K+ channels in mitral dysfunction: mechanically stimulated transcription and hypoxia-induced protein stability? J Am Coll Cardiol 59:390-6 (2012). PubMed: 22133355