Overview

  • Product nameAnti-KIRREL antibody
    See all KIRREL primary antibodies
  • Description
    Rabbit polyclonal to KIRREL
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence (FLEVGTLERY TVERTNSGSG VLSTLTINNV MEADFQTHYN CTAWNSFGPG) of Human KIRREL (NP_060710).(Peptide available as ab109278.)

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab82804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.Can be blocked with KIRREL peptide (ab109278). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionPlays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1.
  • Tissue specificityAbundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylation probably regulates the interaction with NSH2.
    N-glycosylated.
  • Cellular localizationCell membrane. Predominantly located at podocyte slit diaphragm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10845 antibody
    • Kin of IRRE like (Drosophila) antibody
    • Kin of IRRE like antibody
    • Kin of IRRE like protein 1 antibody
    • Kin of IRRE-like protein 1 antibody
    • Kin of irregular chiasm like protein 1 antibody
    • Kin of irregular chiasm-like protein 1 antibody
    • KIRR1_HUMAN antibody
    • Kirrel antibody
    • MGC129542 antibody
    • MGC129543 antibody
    • NEPH 1 antibody
    • NEPH1 antibody
    • Nephrin like protein 1 antibody
    • Nephrin related antibody
    • Nephrin-like protein 1 antibody
    • OTTHUMP00000024055 antibody
    see all

Anti-KIRREL antibody images

  • Anti-KIRREL antibody (ab82804) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab82804 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82804, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-KIRREL antibody (ab82804)

This product has been referenced in:
  • Ito M  et al. Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice. PLoS One 7:e29873 (2012). WB ; Mouse . Read more (PubMed: 22253810) »

See 1 Publication for this product

Product Wall

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending the blocking peptide free of charge on the order ***, which should arrive next week. Please keep me updated abo...

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Thank you so much for your email and the additional information. I think the best way to proceed is the following: Mouse, rat samples and IP have all not been tested with this antibody and are therefore not covered under our guarantee. Bu...

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