Overview

  • Product nameAnti-Kisspeptin antibody
    See all Kisspeptin primary antibodies
  • Description
    Rabbit polyclonal to Kisspeptin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide near the N terminus of the full-length mouse KISS1 protein.

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab19028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 15 kDa.Can be blocked with Kisspeptin peptide (ab37702).

Target

  • FunctionMetastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
  • Tissue specificityVery high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast.
  • Sequence similaritiesBelongs to the KISS1 family.
  • Post-translational
    modifications
    Processed by MMP2 and MMP9.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • KISS 1 antibody
    • KiSS 1 metastasis suppressor antibody
    • Kiss1 antibody
    • KISS1_HUMAN antibody
    • Kisspeptin 1 antibody
    • Kisspeptin 10 antibody
    • Kisspeptin 13 antibody
    • Kisspeptin 14 antibody
    • Kisspeptin antibody
    • Kisspeptin-1 antibody
    • Kisspeptin-10 antibody
    • Kisspeptin-54 antibody
    • Kisspeptin1 antibody
    • Malignant melanoma metastasis suppressor antibody
    • Metastasis suppressor KiSS 1 antibody
    • Metastasis suppressor KiSS1 antibody
    • Metastin antibody
    • MGC39258 antibody
    see all

Anti-Kisspeptin antibody images

  • Anti-Kisspeptin antibody (ab19028) at 1 µg/ml + Jurkat cell lysate (30ug)

    Predicted band size : 15 kDa
    Observed band size : 15,35 kDa (why is the actual band size different from the predicted?)


  • Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Aparna Mitra

    See Abreview

References for Anti-Kisspeptin antibody (ab19028)

This product has been referenced in:
  • Fernandois D  et al. Kisspeptin is involved in ovarian follicular development during aging in rats. J Endocrinol 228:161-70 (2016). Read more (PubMed: 26698566) »
  • Hua W  et al. Analysis of the serum concentrations of kisspeptin and neurokinin B in the geese during reproductive cycle and their localisation in the ovary. Anim Reprod Sci 151:78-84 (2014). Read more (PubMed: 25282553) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Methanol
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Nitsan Kozlovsky

Verified customer

Submitted Oct 01 2012

Thank you for contacting us.
We would be interested in this information. I have therefore generated the testing discount as below:
testing discount: xxxxxx expiration date: 14-Nov-12
The code becomes active once the customer has submitte...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cancer cells)
Loading amount 20 µg
Specification Breast cancer cells
Treatment overexpressing DNAJB6
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Aparna Mitra

Verified customer

Submitted Jul 11 2008

Thank you for your enquiry. I have been informed by our source for this antibody that the proprietary peptide used to generate this antibody is from the N-terminus of the secreted protein, so it will recognize the secreted as well as the intracellular ...

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I'm sorry about the confusion. The NP_839991 (PubMed/Search protein) provides the mouse Kisspeptin amino acid sequence. I've now included this information below. Based on this, amino acid 14-27/the immunogen sequence would be "cvatygeplakvap". ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"