The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionUpon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA.
Tissue specificityWidely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. Contains 1 RRM (RNA recognition motif) domain.