All tags Kits Antibody binding affinities of Protein A, Protein G, Protein L and jacalin

Antibody binding affinities of Protein A, Protein G, Protein L and jacalin

Our guide will help you choose the most suitable antibody binding protein for your antibody purification, immunoprecipitation or chromatin immunoprecipitation experiment.

Antibody binding proteins such as Protein A and Protein G are often used in antibody purification and in applications such as immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP). Each antibody binding protein varies in its ability to bind to antibodies of different subtypes and species. It is therefore important that you match the binding specificity of the immobilized antibody binding protein to the species and antibody subtype of the antibody that you wish to capture. Here, we list the antibody binding properties of Protein A, Protein G, Protein A/G, Protein L and jacalin.

View our beads/resins and columns attached to antibody binding proteins in the table below.

Find out more about affinity purification here.

Antibody binding properties of Protein A, Protein G, Protein A/G and Protein L by antibody species and subtype

Key: +++ strong binding; ++ medium binding; + weak binding; - no binding; ? undetermined

SpeciesAntibody subtypeProtein AProtein GProtein A/GProtein L***
Human

Total IgG
​IgG1
IgG2
​IgG3
​IgG4
​IgM
​IgD
​IgA1
IgA2
Fab
​scFv​

+++
+++
+++
+
+++
+
-
​+
+
+​
+
+++
+++
+++
+++
+++
-
-
-
-
+
-
+++
+++
+++
+++
+++
+
-
+
+
+
+
+++
​+++
+++
+++
+++
+++
+++
+++
+++
+++
+++
MouseTotal IgG
​IgM
​IgG1
​IgG2a
​IgG2b
​IgG3

+++
-
+
+++
+++
+++
+++
-
++
+++
+++
+++
+++
-
++
+++
+++
+++
+++
+++
+++
+++
+++
​+++
RatTotal IgG
IgG1
IgG2a
​​IgG2b
IgG2c

+
+
-
-
+++

++
++
+++
+
+++
++
++
+++
+
+++
+++
+++
+++
+
​+++
RabbitTotal IgG++++++++++
GoatTotal IgG+++++++-
SheepTotal IgG+++++++-
Guinea pigTotal IgG+++++++?
HamsterTotal IgG+++++++++
DonkeyTotal IgG++++++++?
PigTotal IgG++++++++++
DogTotal IgG+++++++?
CatTotal IgG+++++++?
ChickenTotal IgY----
CowTotal IgG+++++++-
HorseTotal IgG+++++++?

***Protein L binding is restricted to antibodies that contain the right subtypes of kappa light chains. Protein L binds to human VκI, VκIII and VκIV subtypes but not to VκII, and only binds to mouse VκI-containing antibodies.


Antibody binding properties of jacalin by antibody species and subtype

SpeciesAntibody subtypeJacalin
HumanTotal IgG
​IgG1
IgG2
​IgG3
IgG4
​IgM
​IgD
​IgA1
​IgA2
​Fab
​scFv
Kappa light chain
Lambda light chain
-
-
-
-
-
-
++
+++
+
+/-
?
-
​-
MouseTotal IgG
​IgA
-
-
RatTotal IgG
​IgA
?
-
RabbitIgA+
GoatIgA-
SheepIgA?
Guinea pigIgA-
HamsterIgA?
DonkeyIgA?
PigIgA-
DogIgA-
CatIgA?
ChickenIgY?
CowIgA-
HorseIgA-

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