All tags Kits Antibody conjugation kits

Antibody conjugation kits

Antibody conjugation kits

The fast way to label your primary antibody

Our antibody conjugation kits allow you to quickly add the label of your choice onto your primary antibody. Choose from labels that include enzymes, fluorochromes, biotin/streptavidin and gold for use in a wide range of immunodetection protocols (flow cytometry, western blot, ELISA, IHC and more).

The antibody conjugation procedure

Our antibody conjugation kits are quick and easy to use:

  • Less than one minute hands-on time;
  • Conjugated antibody ready in ~3 hours when using the regular kits and in less than 20 minutes when using the fast conjugation kits;
  • One-step labeling method with no separation steps.

In contrast to traditional labeling methods, current data shows the antibody conjugation process does not affect the epitope recognition and immunoreactivity of your primary antibody.

Conjugation Process



Tips for sucessful conjugation

The purity and concentration of your antibody are important for successful conjugation. As the antibody labeling chemistry involves free amine groups, any protein or free amine present in the mixture will become conjugated during the conjugation process. The amount of antibody to be labeled per reaction should be 10 µg or more.The antibody should be in 10-50 mM amine-free buffer within a pH range of 6.5-8.5 prior to conjugation. Tris buffer concentrations up to 20mM can be used.

Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our conjugation FAQs.

For optimal conjugation, you may need to purify or concentrate your antibody using our purification and concentration kits.

Characteristics and advantages of conjugation kits

The simple and quick labeling process offered by our antibody conjugation kits enhances your experiments by:

Eliminating:

Indirect detection methods

Column separation steps

Loss of material


Non-specific binding of secondary reagents

Additional incubation steps and sample dilution

Batch-to-batch variation

Complexity associated with other immunoassays
Facilitating:

Faster and reproducible protocols

Study of multi-protein complexes

Advanced multiplex immunoassay applications

Use of antibodies generated from either the same or different species

Identification of best antibody pairs in ELISA















Find out more about the advantages of direct assays.


What other researchers say

"Using the Biotin conjugation kit was very easy and quick. We achieved excellent results in an antibody labeling reaction. The epitope-recognition of the antibody was not affected and detection via streptavidin-HRP is very sensitive"

Researcher, University of Giessen - Germany

"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). Minimal handling and it worked!!"

Researcher, University of California - USA


Selected publications

  • Wang S  et al. Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care. Sci Rep4:3796 (2014).
  • Hashiba K  et al. Galectin-3 contributes to luteolysis by binding to Beta 1 integrin in the bovine corpus luteum. Biol Reprod 91:2 (2014).
  • Jung SM  et al. Constitutive dimerization of glycoprotein VI (GPVI) in resting platelets is essential for binding to collagen and activation in flowing blood. J Biol Chem 287:30000-13 (2012).
  • Wood BA  et al. Domestic cat microsphere immunoassays: Detection of antibodies during feline immunodeficiency virus infection. J Immunol Methods 396:74-86 (2013).
  • Hung LY  et al. Magnetic nanoparticle-based immunoassay for rapid detection of influenza infections by using an integrated microfluidic system. Nanomedicine (2013)

Have you used one of our antibody conjugation kits in a recent publication? Please tell us.