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Our antibody conjugation kits (formerly EasyLink antibody conjugation kits) allow you to rapidly add the label of your choice onto your primary antibody. Choose from labels that include enzymes, biotin/streptavidin, fluorochromes and gold for use in a wide range of immunodetection protocols (Flow cytometry, western blot, ELISA, IHC and more...).
Our antibody conjugation kits allow a quick and easy process with less than one minute hands-on time to label an antibody of your choice, for whatever application you need. The kits offer you the advantage of being a one-step labeling method with no separation steps.
In contrast to traditional labeling methods, current data shows the antibody conjugation process does not affect the epitope recognition and immunoreactivity of your primary antibody.
The purity and concentration of your antibody are important for successful conjugation. As the antibody labeling chemistry involves free amine groups, any protein present in the mixture will become conjugated during the conjugation process - the antibody to be conjugated should be in 10-50mM amine-free buffer within a pH range of 6.5-8.5, modest concentrations of Tris buffer (up to 20mM) are also tolerated. In addition the amount and concentration of the antibody to be labled should be more than or equal to 10µg.
For optimal conjugation you may need to purify or concentrate your antibody using our purification and concentration kits.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemsitry in our conjugation FAQs.
The simple and quick linking process offered by our antibody conjugation kits enhances your experiments by:
|Indirect detection methods|
|Column separation steps|
|Loss of material|
|Non-specific binding of secondary reagents|
|Additional incubation steps and sample dilution|
|Complexity associated with other immunoassays|
Faster and reproducible protocols
Study of multi-protein complexes
Advanced multiplex immunoassay applications
Use of antibodies generated from either the same or different species
Identification of best antibody pairs in ELISA
Find out more about the advantages of direct assays.
"Using the Biotin conjugation kit was very easy and quick. We achieved excellent results in an antibody labeling reaction. The epitope-recognition of the antibody was not affected and detection via streptavidin-HRP is very sensitive"
Researcher, University of Giessen - Germany
"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). Minimal handling and it worked!!"
Researcher, University of California - USA
Have you used one of our antibody conjugation kits in a recent publication? Please tell us.