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Our antibody conjugation kits allow you to quickly add the label of your choice onto your primary antibody. Choose from labels that include enzymes, fluorochromes, biotin/streptavidin and gold for use in a wide range of immunodetection protocols (flow cytometry, western blot, ELISA, IHC and more).
Our antibody conjugation kits are quick and easy to use:
In contrast to traditional labeling methods, current data shows the antibody conjugation process does not affect the epitope recognition and immunoreactivity of your primary antibody.
The purity and concentration of your antibody are important for successful conjugation. As the antibody labeling chemistry involves free amine groups, any protein or free amine present in the mixture will become conjugated during the conjugation process. The amount of antibody to be labeled per reaction should be 10 µg or more.The antibody should be in 10-50 mM amine-free buffer within a pH range of 6.5-8.5 prior to conjugation. Tris buffer concentrations up to 20mM can be used.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our conjugation FAQs.
For optimal conjugation, you may need to purify or concentrate your antibody using our purification and concentration kits.
The simple and quick labeling process offered by our antibody conjugation kits enhances your experiments by:
Indirect detection methods
Column separation steps
Loss of material
Additional incubation steps and sample dilution
Batch-to-batch variationComplexity associated with other immunoassays
Faster and reproducible protocols
Study of multi-protein complexes
Advanced multiplex immunoassay applications
Use of antibodies generated from either the same or different species
Identification of best antibody pairs in ELISA
Find out more about the advantages of direct assays.
"Using the Biotin conjugation kit was very easy and quick. We achieved excellent results in an antibody labeling reaction. The epitope-recognition of the antibody was not affected and detection via streptavidin-HRP is very sensitive"
Researcher, University of Giessen - Germany
"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). Minimal handling and it worked!!"
Researcher, University of California - USA
Have you used one of our antibody conjugation kits in a recent publication? Please tell us.