All tags Kits Antibody conjugation kits

Antibody conjugation kits

Antibody conjugation kits

The fast way to label your primary antibody

Our antibody conjugation kits allow you to rapidly add the label of your choice onto your primary antibody. Choose from labels that include enzymes, biotin/streptavidin, fluorochromes and gold for use in a wide range of immunodetection protocols (Flow cytometry, western blot, ELISA, IHC and more...).

The antibody conjugation procedure:

Our antibody conjugation kits allow a quick and easy process with less than one minute hands-on time to label an antibody of your choice, for whatever application you need. The kits offer you the advantage of being a one-step labeling method with no separation steps.

In contrast to traditional labeling methods, current data shows the antibody conjugation process does not affect the epitope recognition and immunoreactivity of your primary antibody.

Conjugation Process


Tips for sucessful conjugation:

The purity and concentration of your antibody are important for successful conjugation. As the antibody labeling chemistry involves free amine groups, any protein present in the mixture will become conjugated during the conjugation process - the antibody to be conjugated should be in 10-50mM amine-free buffer within a pH range of 6.5-8.5, modest concentrations of Tris buffer (up to 20mM) are also tolerated. In addition the amount and concentration of the antibody to be labled should be more than or equal to 10µg.

For optimal conjugation you may need to purify or concentrate your antibody using our purification and concentration kits.


Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemsitry in our conjugation FAQs.

Characteristics and advantages of conjugation kits:

The simple and quick linking process offered by our antibody conjugation kits enhances your experiments by:

Eliminating:
Indirect detection methods
Column separation steps
Loss of material
Non-specific binding of secondary reagents
Additional incubation steps and sample dilution
Batch-to-batch variation
Complexity associated with other immunoassays
Facilitating:

Faster and reproducible protocols


Study of multi-protein complexes


Advanced multiplex immunoassay applications


Use of antibodies generated from either the same or different species


Identification of best antibody pairs in ELISA

















Find out more about the advantages of direct assays.


What other researchers say:

"Using the Biotin conjugation kit was very easy and quick. We achieved excellent results in an antibody labeling reaction. The epitope-recognition of the antibody was not affected and detection via streptavidin-HRP is very sensitive"

Researcher, University of Giessen - Germany

"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). Minimal handling and it worked!!"

Researcher, University of California - USA


Publications

  • 1. Effects of pH on molecular mechanisms of chitosane-integrin interactions and resulting tight-junction disruptions Hsu LW, et al, Biomaterials, Jan 2013, 34(3):784-793, PMID: 23103155
  • 2. Intraoperative Imaging of Metastatic Lymph Nodes Using a Fluorophore-conjugated Antibody in a HER2/neu-expressing Orthotopic Breast Cancer Mouse Model Wu J et al, Anticancer Res, Feb 2013, 33(2):419-24, PMID: 23393332
  • 3. APR-246/PRIMA-1MET rescues epidermal differentiation in skin keratinocytes derived from EEC syndrome patients with p63 mutations Shen J, et al, Proc Natl Acad USA, Feb 2013, 110(6):2157-62, PMID: 23355676
  • 4. Constitutive dimerization of glycoprotein VI (GPVI) in resting platelets is essential for binding to collagen and activation in flowing blood. Jung SM, et al, J Biol Chem, Aug 2012, 287(35):30000-13, PMID: 22773837

Have you used one of our antibody conjugation kits in a recent publication? Please tell us.