All tags IHC Antigen retrieval reagents

Antigen Retrieval Reagents

Antigen retrieval reagents

In order to reverse the antigen masking effects of formaldehyde fixation either Proteolytic-Induced Epitope Retrieval (PIER) or Heat-Induced Epitope Retrieval (HIER) can be used.

The preferred technique is dependent on tissue, fixation and/or primary antibodies you intend to use. An initial recommendation is to test two methods from HIER and one or two methods from PIER, to identify what would be best for your tissue sample.

Antigen retrieval may not be required for frozen cross section as cross links are formed through formalin fixation. To help you optimally reveal your target antigens we offer a selection of products allowing you to use less antibody and produce superior staining in your immunohistochemistry (IHC).

Heat-Induced Epitope Retrieval (HIER)

Product nameSize/descriptionProduct code
Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0)

125mL

250mL

ab93678

ab94674

Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0)

125mL

250mL

ab93680

ab94677

Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)

125mL

250mL

ab93684

ab94681

Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0)

125mL

250mL

ab93682

ab94680
Heat Mediated High pH 9.5 Antigen Retrieving Solution1Lab972
10x Heat Mediated Antigen Retrieval Solution pH 6.0250mLab973
10X Tris-HCl Buffer for HIER50mLab128986


Proteolytic-Induced Epitope Retrieval (PIER)

Product nameSize/descriptionProduct code
HistoReveal15mLab103720
Pepsin Solution7mLab64201
Pepsin Solution15mL, 125mLab128991
Proteinase K4mLab64220
Trypsin Enzymatic Pretreatment7mLab64205
Trypsin Enzymatic Antigen Retrieval Solution1.6mL concentrated liquid trypsin and 25ml trypsin bufferab970


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