All tags IHC Chromogens and enhancers

IHC and ISH chromogens and enhancers

Chromogens and enhancers

Chromogens/substrates and enhancers localize and increase the staining intensity of tissue specimens stained in histochemical methods.

Typically the enzymes horseradish peroxidase (HRP) and alkaline phosphatase (AP) are used together with the appropriate chromogen/substrate to produce a reaction product that can generally be visualized using light microscopy. For histochemical detection using gold conjugated primary or secondary antibodies in electron microscopy, silver enhancer reagents can be used to aid visualization of bound antibody.

Peroxidase based chromogens and enhancers

Suitable for horesradish peroxidase (HRP) based detection methods.

ab103723-SteadyDABPlus

Steady DAB/Plus (ab103723) – 200mL

  • More sensitive than traditional DAB solutions
  • Stable for 2 weeks after dilution
  • Ideal for high-throughput use
ab169561-StayYellow

StayYellow/HRP (Alcohol and Xylene compatible) (ab169561) – 30mL

  • Easily distinguished from other stains
  • Can be used in IHC or ISH
ab179917-StayBlack

StayBlack/HRP (ab179917) – 30mL, 110mL

  • Can be used in IHC or ISH
  • Suitable for both automation and manual use

Phosphatase based chromogens and enhancers

Suitable for alkaline phosphatase (AP) based detection methods.

stayredap-plus-chromogen-ab176914

StayRed/AP Plus (ab176914) – 30mL, 110mL

  • Can be used in IHC or ISH
  • Insoluble in organic solvents
  • Compatible with automation
stayredAP-chromogen-ab103741

StayRed/AP (Alcohol and Xylene compatible) (ab103741) – 30mL

  • Can be used in IHC or ISH
  • Ideal for manual staining
staygreenAP-chromogen-ab156428

StayGreen/AP (Alcohol and Xylene compatible) (ab156428) – 30mL

  • Distinguishable color
  • Insoluble in organic solvents
  • Can be used in IHC or ISH
stayblueap-plus-chromogen-ab176915

StayBlue/AP Plus (ab176915) – 30mL, 110mL

  • Can be used in IHC or ISH
  • Insoluble in organic solvents
  • Compatible with automation
stayblueap-chromogen-ab178453

StayBlue/AP (Alcohol and Xylene compatible) (ab178453) – 30mL

  • Can be used in IHC or ISH
  • Insoluble in organic solvents
  • Ideal for manual staining