Organelle visualization with organelle-selective stains or dyes is a key tool in fluorescence imaging of cells and tissues. These specific stains are suitable counterstains to antibodies to help the identification of location-specific targets of interest within the cell.
|Structure||Dye Information||Live/fixed (cells or tissue)|
|Endoplasmic reticula, smooth and rough (ER)||Cell-permeable dye that localizes to endoplasmic reticula.||Live/fixed (aldehyde)|
|Golgi apparatus||Cell-permeable dye that localizes to Golgi.||Live/fixed (aldehyde)|
|F-actin / actin filaments||Phalloidin-conjugated dye that binds to actin filaments but not to actin monomers or dimers.||Fixed (aldehyde)|
|Lysosome||Cell-permeable dye that accumulates in the lysosome via the lysosome pH gradient (pH 4.5 - 4.8).||Live (can be fixed in aldehyde after staining)|
|Mitochondria||Cell-permeable dye that accumulates in the mitochondria via the mitochondrial membrane potential.||Live (can be fixed in aldehyde after staining)|
|Nucleolus||Cell-permeable dye that accumulates in the nucleolus.||Live|
Actin filament staining (red, ab112127) in Hydractinia, a colonial marine hydroid. Nuclei are shown in blue (Hoechst) for counterstaining.
Image courtesy of an Abreview.
Analyze your cells of interest for hours or days thanks to our cell tracking dyes that are retained in living cells. These dyes are hydrophobic compounds that can permeate through the plasma membrane and become strongly fluorescent once inside the cell.
|Product Range||Description||Best used for|
|Live cell labeling||Non-fluorescent dye with a cell-retaining moiety. Upon entering cells, dye becomes fluorescent and trapped in the cells.||Cell adhesion, cellular motility and cell viability studies. Cells need to be viewed in less than 2 hours.|
|Live cell tracking||Non-fluorescent dye with a cell-retaining moiety. Upon entering cells, dye becomes fluorescent and trapped in cells. Compatible with cell culture medium (no washing step).||Multicolor analysis of cells for cell drug resistance, cell adhesion and chemotaxis. Cells need to be viewed in less than 24 - 48 hours as dye will not pass to daughter cells.|
|Cell proliferation||Non-fluorescent dye with a cell-retaining moiety. Upon entering cells, dye becomes fluorescent and trapped in cells.|
The adduct formed in labeled cells is inherited by daughter cells after cell division.
|Analysis of heterogeneous cell populations over time. Fluorescence is preserved upon formaldehyde fixation. Dye is retained for up to 9 generations after staining.|
|Fixable cell viability||Impermeable live-cell dye that only enters cells with compromised membranes. Viable cells show low fluorescence while non-viable cells show intense staining.||Cell viability evaluation in a multiplex experiment. Fluorescence is preserved upon formaldehyde fixation.|