ELISA kits to mouse targets are often less reproducible, specific, and sensitive compared to other species, due to the use of either batch-specific polyclonal antibodies or mouse monoclonal antibodies.
Our ELISA kits are different: unlike our competitors, we use only rabbit monoclonal antibodies in our antibody pairs to mouse targets to reduce non-specific signal due to native mouse Ig, resulting in less cross-reactivity in mouse or rat.
Superior sensitivity compared with non-monoclonal antibodies
Mouse IL-1a SimpleStep® ELISA kit (ab199076) made with a pair of rabbit monoclonal antibodies (ab210893) shows superior performance in sensitivity and assay precision over a standard ELISA kit made with non-rabbit monoclonal antibodies.
|Product||Range (pg/mL)||Sensitivity (pg/mL)||Intra-assay precision||Inter-assay precision|
Mouse IL-1a SimpleStep ELISA kit (ab199076)
|Mouse IL-1a ELISA kit (ab113344)||12.23–3000||10||10%||10%|