All tags elisa-matched-antibody-pairs-and-multiplex-immunoassays ELISA kits

ELISA kits

ELISA kits

Fully validated and ready-to-use enzyme-linked immunosorbent assay (ELISA) kits.

Choose from our wide range of ELISA kits to detect or quantitate one or more specific proteins in a solution or biological sample.

Figure 1. Different ELISA formats. 

Different ELISA formats

Sandwich ELISA

In a sandwich ELISA assay, an immobilized capture antibody pre-coated onto a 96-well plate binds to the target protein and captures it from a liquid sample.

Detection antibodies are then added to create complexes where the detection antibodies also bind to the captured target protein, catalyze the appearance of a colored or fluorescent product through the action of a covalently linked enzyme.

The amount of color produced is proportional to the amount of target protein in the sample. A sandwich ELISA can be:

SimpleStep ELISA Kits

Our brand of ELISA kits provide improved speed and performance while retaining the familiar process and standard data output of a traditional ELISA kit.

SimpleStep ELISA kits reduce the number of wash steps from 9 (3x3) to 3 (1x3) by enabling complex formation in one step rather than sequentially. Total time required is less than two hours.

We also offer ELISA sets with validated antibody pairs and standards for those wanting to develop their own ELISA method.

Discover the benefits of SimpleStep ELISA kits

Competitive ELISA

In a competitive ELISA, sample antigen and labeled antigen compete for capture antibody binding. The more target protein there is in the sample, the less labeled antigen will be captured and the weaker the signal.

  • Corticosterone (CSCI) ELISA kit (ab108821)
    • Biotinylated corticosterone competes with corticosterone in the sample for binding to the capture antibody. The signal intensity is inversely proportional to the unlabeled corticosterone in the sample.

Indirect ELISA

In an indirect ELISA, the target protein adheres to the bottom of a 96-well plate, a primary antibody binds to the target, and a labeled secondary antibody binds to the primary for detection. The method can also be used to detect specific antibodies in a serum sample by substituting the serum for the primary antibody.

The main advantage of an indirect ELISA is that one labeled secondary antibody can be used with many different primary antibodies.

Example validation data

Detection of frataxin in HeLa cells

Figure 2. Detection of frataxin in HeLa cells with Frataxin ELISA kit (ab115346). Sensitivity = 0.3 ng/mL, intra-assay precision = 6% CV, inter-assay precision = 9.9% CV.

Determination of the levels of frataxin in human samples

Figure 3. Determination of the levels of frataxin in human samples. Transformed B lymphocyte cells from Friedreich's Ataxia samples are compared to heterozygous carrier B-lymphocyte cells and control B-lymphocyte cells. B lymphocyte extracts were analyzed at 0.2 mg/mL (10 µg/well) and frataxin concentrations were interpolated from the standard curve.

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