All tags ELISA Mouse SimpleStep ELISA® Kits

Mouse SimpleStep ELISA® Kits

SimpleStep ELISA Kits use RabMAb® monoclonal antibodies to mouse targets for enhanced assay performance.

The Problem:

ELISA kits to mouse targets may be less reproducible, specific and sensitive when compared to ELISA kits to other species’ targets due to the use of batch-specific polyclonal antibodies. Monoclonal antibodies are preferred for consistent ELISA performance; however, the use of a mouse monoclonal antibody to mouse targets may generate an unacceptable level of background resulting in a less sensitive assay.

The Solution:

Many SimpleStep ELISA kits to mouse targets use RabMAb® antibody technology, Abcam’s patented rabbit monoclonal technology, to achieve greater sensitivity and specificity. Rabbit monoclonal antibodies have the enhanced affinity of a rabbit host antibody coupled with the specificity that is the hallmark of monoclonal antibodies. Most RabMAb antibodies are recombinant which means that greater batch-to-batch consistency in quality and performance. 

During the development of a SimpleStep ELISA, multiple rabbit monoclonal antibody clones are screened for optimal performance as both capture and detector antibodies.  As a result, SimpleStep ELISA  frequently exhibit improved assay performance with greater sensitivity than the leading competitors:

Figure 1. Mouse IL-5 SimpleStep ELISA kit (ab204523) is more sensitive than the leading competitor.

Figure 2. Mouse IL-1 beta SimpleStep ELISA kit (ab197742) is more sensitive than the leading competitor.

In addition to improvements in sensitivity, SimpleStep ELISA kits offers these additional benefits to the researcher:

  • Validated for complex sample types like serum and plasma so that you can be confident that it will accurately quantify your target.
  • Broad dynamic range reduces the need for multiple sample dilutions allowing you to measure more samples per plate.
  • Fast results are achieved because the antibody and analyte complex form in one step in the well which allows the assay to be completed in only 90 minutes with equivalent or superior performance to a conventional ELISA.​

In addition to improvements in sensitivity, SimpleStep ELISA kits offers these additional benefits to the researcher:

Validated for complex sample types like serum and plasma so that you can be confident that it will accurately quantify your target.

Broad dynamic range reduces the need for multiple sample dilutions allowing you to measure more samples per plate.

Fast results are achieved because the antibody and analyte complex form in one step in the well which allows the assay to be completed in only 90 minutes with equivalent or superior performance to a conventional ELISA.


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