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FirePlex immunoassays feature fully customizable multiplex panels that allow you to analyze up to 75 analytes per well using 12.5 µL of sample. The assays use our unique porous FirePlex particles and high-performance recombinant monoclonal antibody pairs to provide up to a 5-log dynamic range and 0.5 pg/mL assay sensitivity. FirePlex assays are validated with sample types like plasma, serum, and urine, and can be either run on your own flow cytometer or samples can be sent for analysis in our service lab.
The FirePlex immunoassay is run in a 96-well filter plate, streamlining wash steps using a vacuum manifold. The multiplex assay run-time is 2.5 hours followed by flow cytometry using a compatible flow cytometer. Our unique three-region particle encoding uses a combination of fluorescent dyes to distinguish individual analytes and to quantify the amount of analyte present. The FirePlex particles are also used in our multiplex miRNA assays, thus enabling analysis of miRNA and protein analytes on the same platform.
The sensitivity and broad dynamic range of the FirePlex immunoassays enable you to quantify both low and high abundance analytes without needing to run multiple sample dilutions – saving precious sample.
Sensitivity benchmarking. FirePlex cytokine assay sensitivity for 44 human and 50 mouse cytokines was benchmarked against the sensitivity stated on the assay datasheet for standard sensitivity Luminex assays from the four leading suppliers. Sensitivity data for equivalent analytes was not always publicly available and was collated across the four suppliers.
Dynamic range benchmarking. The dynamic range for FirePlex assays for 15 key human cytokines (red) was benchmarked (based on standard curve data on assay datasheets) against Luminex assays from the four leading suppliers. Mean range and standard deviation are shown.
FirePlex assays give you the freedom to build your perfect multiplex panel.
Comparison of the number of cytokine analytes available for FirePlex Immunoassays vs competitors (A and B = bead-based assays for flow cytometers, c = proprietary multiplex array). *For FirePlex multiplex assays, red bars indicate currently available analytes and pink bars indicate additional analytes available in April 2017 for human cytokines and July 2017 for mouse cytokines.
FirePlex immunoassays can be conveniently run on your own existing flow cytometer, eliminating the need to purchase dedicated equipment. FirePlex particles are validated for use with the following flow cytometers fitted with green, yellow and red detectors.
Don't have access to a compatible flow cytometer? You can still profile cytokines with the help of our sample profiling team.
FirePlex moves beyond traditional multiplex assays that require dedicated equipment and offer you a broad range of analytes without ever compromising sensitivity or dynamic range.
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FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.