All tags Primary antibodies Tetramethylbenzidine (TMB) and assay buffers

Tetramethylbenzidine (TMB) and assay buffers

Blocking buffers and reagents to support your own immunoassay development including blocking, stability and detection reagents.

Additionally, we offer TMB (3,3’,5,5’-Tetramethylbenzidine), the most popular chromogenic substrate for HRP detection with different kinetic formulations to help optimize your particular application.

Different kinetic TMB formulations

The choice of the TMB substrate formulation optimizes the ELISA dynamic range as well as the assay sensitivity.

  • Detection of low concentration proteins of interest over a short period of time
  • Improved assay sensitivity and performance
  • Reliable standard curve

Table 1: Range of TMB substrates with different kinetic formulations

ProductFunctionProduct code
TMB ELISA substrate (Highest Sensitive)Most sensitive TMB formulationab171522
TMB ELISA substrate (High Sensitivity)Most popular and robust formulationab171524
TMB ELISA substrate (Slow Kinetic Rate)Allow a wide dynamic/working range without compromising the assay sensitivity   ab171525
TMB ELISA substrate (Slower Kinetic Rate)Option for maximum dynamic/working rangeab171526
TMB ELISA substrate (Slowest Kinetic Rate)Option for extended dynamic range and improved reproducibility   ab171527


TMB family kinetics

Figure 1: View the kinetics among different TMB formulations after a sandwich ELISA where the capture antibody was blocked with Immunoassay Blocking Buffer (ab171534) and the HRP conjugated detection antibody was diluted with HRP Stability Conjugate Reagent (ab171537).

Blocking buffers and conjugate stabilizers

The buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state when dried or in solution ensuring the biological activity of the protein.

Table 2: Buffers to preserve the conformational spatial arrangement of proteins.

ProductStabilizationELISAWBMicro-spheres
Immunoassay Blocking Buffer  
ab171534
xxx
Immunoassay Blocking and Protein Stabilizer (BSA free)
ab171535
xxxx
AP Stability Conjugate Reagent  ab171536
x

HRP Stability Conjugate Reagent   ab171537
x

See below some comparative studies when using our buffers with monoclonal or polyclonal antibodies.

ab171534 stability

Figure 2: After 30 months in Immunoassay Blocking Buffer (ab171534) at room temperature, the monoclonal antibody retained 70% activity and the polyclonal antibody retained 95% activity.

ab171535 stability

Figure 3: After 30 months in Immunoassay Blocking and Protein Stabilizer (BSA free) (ab171535) at room temperature, the monoclonal antibody retained greater than 85% activity and the polyclonal antibody retained 90% activity.

Table 3: Blocking and conjugate buffers guide.

ProductFunction
Immunoassay Blocking Buffer   - ab171534Blocking agent that can be dried down or used for same-day assays. Preserves the conformation and activity of dried antibodies, proteins or enzymes coated to a surface
Immunoassay Blocking Buffer (BSA free) - ab171535Blocking solution without BSA. Synthetic formulation to eliminate background issues
AP Stability Conjugate Reagent - ab171536Preserves AP conjugated proteins in native folded state in solution
HRP Stability Conjugate Reagent   - ab171537Preserves HRP conjugated proteins in native folded state in solution


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