Additionally, we offer TMB (3,3’,5,5’-Tetramethylbenzidine), the most popular chromogenic substrate for HRP detection with different kinetic formulations to help optimize your particular application.
The choice of the TMB substrate formulation optimizes the ELISA dynamic range as well as the assay sensitivity.
Table 1: Range of TMB substrates with different kinetic formulations
|TMB ELISA substrate (Highest Sensitive)||Most sensitive TMB formulation||ab171522|
|TMB ELISA substrate (High Sensitivity)||Most popular and robust formulation||ab171524|
|TMB ELISA substrate (Slow Kinetic Rate)||Allow a wide dynamic/working range without compromising the assay sensitivity||ab171525|
|TMB ELISA substrate (Slower Kinetic Rate)||Option for maximum dynamic/working range||ab171526|
|TMB ELISA substrate (Slowest Kinetic Rate)||Option for extended dynamic range and improved reproducibility||ab171527|
The buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state when dried or in solution ensuring the biological activity of the protein.
Table 2: Buffers to preserve the conformational spatial arrangement of proteins.
|Immunoassay Blocking Buffer |
|Immunoassay Blocking and Protein Stabilizer (BSA free)|
|AP Stability Conjugate Reagent ab171536||x|
|HRP Stability Conjugate Reagent ab171537||x|
See below some comparative studies when using our buffers with monoclonal or polyclonal antibodies.
Figure 2: After 30 months in Immunoassay Blocking Buffer (ab171534) at room temperature, the monoclonal antibody retained 70% activity and the polyclonal antibody retained 95% activity.
Figure 3: After 30 months in Immunoassay Blocking and Protein Stabilizer (BSA free) (ab171535) at room temperature, the monoclonal antibody retained greater than 85% activity and the polyclonal antibody retained 90% activity.
Table 3: Blocking and conjugate buffers guide.
|Immunoassay Blocking Buffer - ab171534||Blocking agent that can be dried down or used for same-day assays. Preserves the conformation and activity of dried antibodies, proteins or enzymes coated to a surface|
|Immunoassay Blocking Buffer (BSA free) - ab171535||Blocking solution without BSA. Synthetic formulation to eliminate background issues|
|AP Stability Conjugate Reagent - ab171536||Preserves AP conjugated proteins in native folded state in solution|
|HRP Stability Conjugate Reagent - ab171537||Preserves HRP conjugated proteins in native folded state in solution|