Anti-KLB antibody (ab106794)
Key features and details
- Rabbit polyclonal to KLB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLB antibody
See all KLB primary antibodies -
Description
Rabbit polyclonal to KLB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Macaque monkey, Gorilla -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in HepG2 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. -
Sequence similarities
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. -
Domain
Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 152831 Human
- Omim: 611135 Human
- SwissProt: Q86Z14 Human
- Unigene: 90756 Human
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Alternative names
- Beta-klotho antibody
- BetaKlotho antibody
- BKL antibody
see all
Images
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Anti-KLB antibody (ab106794) at 1 µg/ml + HepG2 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab106794 has been referenced in 11 publications.
- Guo Z et al. β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 36232594
- Panera N et al. The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation. EBioMedicine 65:103249 (2021). PubMed: 33640795
- Xie T et al. The potential role of plasma fibroblast growth factor 21 as a diagnostic biomarker for abdominal aortic aneurysm presence and development. Life Sci 274:119346 (2021). PubMed: 33713667
- Chen T et al. ßKlotho, a direct target of miR-206, contributes to the growth of hepatoblastoma through augmenting PI3K/Akt/mTOR signaling. Am J Cancer Res 11:1982-2004 (2021). PubMed: 34094665
- Mao C et al. ßKlotho Inhibits Cell Proliferation by Downregulating ELK4 and Predicts Favorable Prognosis in Prostate Cancer. Cancer Manag Res 13:6377-6387 (2021). PubMed: 34408497