Overview

  • Product nameAnti-KLB antibody
  • Description
    Rabbit polyclonal to KLB
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human KLB.

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.

Properties

Applications

Our Abpromise guarantee covers the use of ab106794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionContributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-klotho antibody
    • BetaKlotho antibody
    • BKL antibody
    • KLB antibody
    • KLOTB_HUMAN antibody
    • Klotho beta like protein antibody
    • Klotho beta-like protein antibody
    see all

Anti-KLB antibody images

  • Anti-KLB antibody (ab106794) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-KLB antibody (ab106794)

ab106794 has not yet been referenced specifically in any publications.

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