Overview

  • Product nameAnti-KLC1 antibody
    See all KLC1 primary antibodies
  • Description
    Rabbit polyclonal to KLC1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 523-573 of Human Kinesin 2 (NP_891553.2).

  • Positive control
    • HeLa and 293T cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
  • Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
  • Sequence similaritiesBelongs to the kinesin light chain family.
    Contains 6 TPR repeats.
  • Post-translational
    modifications
    Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kinesin 2 60/70kDa antibody
    • Kinesin light chain 1 antibody
    • Kinesin2 antibody
    • KLC 1 antibody
    • KLC antibody
    • Klc1 antibody
    • KLC1_HUMAN antibody
    • KNS 2 antibody
    • KNS 2A antibody
    • KNS2 antibody
    • KNS2A antibody
    • Medulloblastoma antigen MU MB 2.50 antibody
    • MGC15245 antibody
    see all

Anti-KLC1 antibody images

  • All lanes : Anti-KLC1 antibody (ab95882) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 65 kDa


    Exposure time : 30 seconds
  • ab95882 at 0.4 µg/ml detecting Kinesin 2 in HeLa whole cell lysate by WB following IP.
    Lane 1: Kinesin 2 with 3 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an upstream epitope of Kinesin 2
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds

References for Anti-KLC1 antibody (ab95882)

ab95882 has not yet been referenced specifically in any publications.

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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab95882 has never been tested with samples from human.

All tested species covered by the 6 month guarantee are stated on our datasheets, an...

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