Overview

  • Product nameAnti-KLC1 antibody [L2]
    See all KLC1 primary antibodies
  • Description
    Mouse monoclonal [L2] to KLC1
  • SpecificityReacts with the light chain of kinesin near the C-terminal.
  • Tested applicationsSuitable for: WB, IP, ICC, RIAmore details
    Unsuitable for: ICC/IF or IHC
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length protein (Cow). Bovine brain kinesin

  • Positive control
    • mouse brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab23586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 65-70 kDa (predicted molecular weight: 61 kDa). Do not dilute in buffers containing any other protein or detergent. See reference Lazarov O et al reference.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.
  • Application notesIs unsuitable for ICC/IF or IHC.
  • Target

    • FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
    • Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
    • Sequence similaritiesBelongs to the kinesin light chain family.
      Contains 6 TPR repeats.
    • Post-translational
      modifications
      Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Kinesin 2 60/70kDa antibody
      • Kinesin light chain 1 antibody
      • Kinesin2 antibody
      • KLC 1 antibody
      • KLC antibody
      • Klc1 antibody
      • KLC1_HUMAN antibody
      • KNS 2 antibody
      • KNS 2A antibody
      • KNS2 antibody
      • KNS2A antibody
      • Medulloblastoma antigen MU MB 2.50 antibody
      • MGC15245 antibody
      see all

    References for Anti-KLC1 antibody [L2] (ab23586)

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    I'm delighted to be able to confirm that the correct band size for Kinesin Light Chain is actually 65-70kDa and that you are therefore seeing the correct band now. I apologize for the error on the datasheet, this has been corrected immediately. I h...

    Read More

    Thank you for your reply. I would recommend to incubate the membrane in 5%BSA (in TBST Tween20 0.1%) for 30min-1hour, then rinsing lightly (5 seconds) in TBST, and incubating the primary in TBST only.This should block well but not excessively. I hope t...

    Read More

    I have been informed today by the source of ab23586 that the positive control recommended for this antibody is brain lysate, as this tissue has the highest levels. The antibody should not be diluted in buffers containing any other protein or detergent...

    Read More

    Thank you for your reply and expressing your concern regarding a possible delay in the shipping of the vial. This could indeed be a possible reason for a damaged vial. However the only order in the US for this vial, to Yale, was order 115334 and this w...

    Read More

    I'm very sorry to hear you are experiencing a problem with ab23586. I am trying to find out from the source of this antibody what the recommended positive control is as I am concerned that your samples may not have high levels of kinesin light chai...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"