KLC1 overexpression 293T lysate (whole cell) (ab94276)

Overview

  • Product name
    KLC1 overexpression 293T lysate (whole cell)
  • Description
    KLC1 overexpression lysate
  • General notes
    ab94276 is a 293T cell transfected lysate in which Human Kinesin 2 has been transiently over-expressed using a pCMV-Kinesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Tissue specificity: Found in a variety of tissues. Mostly abundant in brain and spine. Similarity: Belongs to the kinesin light chain family. Contains 6 TPR repeats. PTM: Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.

Applications

Our Abpromise guarantee covers the use of ab94276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94276 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-KLC1 antibody (ab88095) at 1/500 dilution

    Lane 1 : KLC1 overexpression 293T lysate (whole cell) (ab94276)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94276 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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