Overview

  • Product name
  • Description
    Rabbit polyclonal to KLC2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human KLC2 (NP_001128247.1).

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified using an epitope specific to KLC2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
  • Sequence similarities
    Belongs to the kinesin light chain family.
    Contains 6 TPR repeats.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kinesin light chain 2 antibody
    • KLC 2 antibody
    • KLC2 antibody
    • KLC2_HUMAN antibody

Images

  • All lanes : Anti-KLC2 antibody (ab95881) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 3 minutes
  • ab95881 at 0.4 µg/ml detecting KLC2 in HeLa whole cell lysate by WB following IP.
    Lane 1: KLC2 with 3 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes a downstream epitope of KLC2
    Lane 3: control IgG
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:
  • Carpentier DC  et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog 11:e1004723 (2015). WB, IF . Read more (PubMed: 25760349) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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