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Rabbit monoclonal [EPR11149(2)] to KLC3
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLC3 aa 350-450. The exact sequence is proprietary.
Database link: Q6P597
Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
1/100 - 1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Belongs to the kinesin light chain family.
Contains 5 TPR repeats.
The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding.
Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.
Information by UniProt
Kinesin light chain 2 antibody
Kinesin light chain 3 antibody
Western blot - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution Lane 1 : Jurkat cell lysate Lane 2 : U87-MG cell lysate Lane 3 : HepG2 cell lysate Lane 4 : BxPC-3 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
Immunoprecipitation - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
Flow Cytometry - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"