Anti-KLF10 antibody (ab73537)
Key features and details
- Rabbit polyclonal to KLF10
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-KLF10 antibody
See all KLF10 primary antibodies -
Description
Rabbit polyclonal to KLF10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Non human primates -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human KLF10.
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Positive control
- This antibody gave a positive signal in the following lysates: Jurkat Whole Cell; Ramos Whole Cell; Human Skeletal Muscle Tissue; Human Brain Tissue; Mouse Liver Tissue; Mouse Testis Tissue and Rat Thymus Tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7071 Human
- Entrez Gene: 21847 Mouse
- Entrez Gene: 81813 Rat
- Omim: 601878 Human
- SwissProt: Q13118 Human
- SwissProt: O89091 Mouse
- SwissProt: O08876 Rat
- Unigene: 435001 Human
see all -
Alternative names
- Early growth response, alpha antibody
- Early growth response, alpha like antibody
- EGR A antibody
see all
Images
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All lanes : Anti-KLF10 antibody (ab73537) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Testis (Mouse) Tissue Lysate
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab73537 has been referenced in 7 publications.
- Yang Y et al. Effect of Photodynamic Therapy on Gemcitabine-Resistant Cholangiocarcinoma in vitro and in vivo Through KLF10 and EGFR. Front Cell Dev Biol 9:710721 (2021). PubMed: 34805140
- Wara AK et al. KLF10 Deficiency in CD4+ T Cells Triggers Obesity, Insulin Resistance, and Fatty Liver. Cell Rep 33:108550 (2020). PubMed: 33378664
- Yang GS et al. Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. Theranostics 10:7787-7811 (2020). PubMed: 32685020
- Li H et al. Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression. EMBO Mol Med 11:e10835 (2019). PubMed: 31709724
- Lin CL et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med 11:N/A (2019). PubMed: 30948420
- Hung MS et al. Knockdown of cullin 4A inhibits growth and increases chemosensitivity in lung cancer cells. J Cell Mol Med 20:1295-306 (2016). WB ; Human . PubMed: 26969027
- Peng DJ et al. Noncanonical K27-linked polyubiquitination of TIEG1 regulates Foxp3 expression and tumor growth. J Immunol 186:5638-47 (2011). WB ; Human . PubMed: 21471442