The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).
FunctionRepresses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells.
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
DomainThe Ala/Pro-rich domain may contain discrete activation and repression subdomains and also can mediate protein-protein interactions.