Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF15
  • Specificity
    ab22851 reacts with Krueppel-like factor 15 (KLF15).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 29-83 of Human KLF15

  • Positive control
    • HepG2 cell lysate and human liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab22851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator. Binds to the GA element of the CLCNKA promoter.
  • Tissue specificity
    Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kidney enriched Kruppel like factor antibody
    • Kidney-enriched krueppel-like factor antibody
    • KKLF antibody
    • KKLF protein antibody
    • KLF 15 antibody
    • KLF15 antibody
    • KLF15_HUMAN antibody
    • Krueppel-like factor 15 antibody
    • Kruppel like factor 15 antibody
    see all

Images

  • Anti-KLF15 antibody (ab22851) at 4 µg/ml + HepG2 cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling KLF15 with ab22851 at a concentration of 4-8µg/ml. Positive stained hepatocytes marked by arrows. Magnification X400.

References

ab22851 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Sheep Tissue lysate - whole (skeletal muscle tissue)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
50 µg
Specification
skeletal muscle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Sep 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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