Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF17
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: YGRPQAEMEQ EAGELSRWQA AHQAAQDNEN SAPILNMSSS SGSSGVHTSW , corresponding to a region within N terminal amino acids 2 - 51 of Human KLF17 (NP_775755)

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ40160 antibody
    • KLF17 antibody
    • KLF17_HUMAN antibody
    • Krueppel like factor 17 antibody
    • Krueppel-like factor 17 antibody
    • Novel zinc finger protein antibody
    • RP4 675G8.1 antibody
    • RP4675G8.1 antibody
    • Zfp393 antibody
    • Zinc finger protein 393 antibody
    • ZNF393 antibody
    see all

Images

  • Anti-KLF17 antibody (ab84196) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

This product has been referenced in:
  • Dong P  et al. Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 24220291) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Revelation (citrate ph6.2)
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted May 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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