Overview

  • Product nameAnti-KLF2 antibody
    See all KLF2 primary antibodies
  • Description
    Goat polyclonal to KLF2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide: ALSEPILPSFST, corresponding to N terminal amino acids 2-13 of Human KLF2.

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab17008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesELISA: 1/32000.
    WB: Use at a concentration of 1.5 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa) from human heart lysates.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the CACCC box in the beta-globin gene promoter and activates transcription.
    • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications
      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • KLF 2 antibody
      • Klf2 antibody
      • KLF2_HUMAN antibody
      • Krueppel-like factor 2 antibody
      • Kruppel like factor 2 antibody
      • Kruppel like factor antibody
      • Kruppel like factor LKLF antibody
      • Kruppel-like factor 2 (lung) antibody
      • Lklf antibody
      • Lung krueppel like factor antibody
      • Lung krueppel-like factor antibody
      • Lung Kruppel like zinc finger transcription factor antibody
      see all

    Anti-KLF2 antibody images



    • Predicted band size : 41 kDa


      ab17008 at 1.5µg/ml detecting KLF2 from human heart lysate by Western blot. 35µg of total protein was loaded per lane. Primary antibody was incubated for 1 hour. The blot was detected using chemiluminescence.

    References for Anti-KLF2 antibody (ab17008)

    This product has been referenced in:
    • Tuomisto TT  et al. Simvastatin has an anti-inflammatory effect on macrophages via upregulation of an atheroprotective transcription factor, Kruppel-like factor 2. Cardiovasc Res 78:175-84 (2008). Read more (PubMed: 18192240) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. Since this KLF2 is a transcription factor, it is expected to reside in the nucleus. Therefore, it will recognize the intracellular domain. I hope this information will be helpful. If there is anything else that I ...

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    Thank you for your enquiry. No, this has not been specifically tested. The sequence of the peptide used as the immunogen is listed on the online datasheet and you can BLAST it (the BLAST link is on the online datasheet too) to see if it is likely to cr...

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    Thank you for taking the time to send us your protocol details, this was very useful for me to understand your problem. We have not tested this antibody in the same samples as yours; in our hands, our positive control of human heart lysate gave a s...

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    Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with ab17008. Would you be able to provide me with a detailed protocol? This will assist in helping to troubleshoot. It would be really helpful if you could cl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"