Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 153-334 of Human KLF3 (UniProt P57682).

  • Positive control
    • A549 and HCT116 cell lysates; SCC4 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Sumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic krueppel-like factor antibody
    • Basic Kruppel like factor antibody
    • BKLF3 antibody
    • CACCC box binding protein BKLF antibody
    • CACCC-box-binding protein BKLF antibody
    • Klf3 antibody
    • KLF3_HUMAN antibody
    • Krueppel-like factor 3 antibody
    • Kruppel like factor 3 (basic) antibody
    • MGC48279 antibody
    • TEF-2 antibody
    • TEF2 antibody
    • Transcript ch138 antibody
    • Zinc finger protein 741 antibody
    • ZNF741 antibody
    see all

Images

  • Anti-KLF3 antibody (ab154531) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 39 kDa
  • Immunohistochemical analysis of paraffin-embedded SCC4 xenograft tissue labeling KLF3 with ab154531 at 1/100 dilution.

References

This product has been referenced in:
  • Wang X  et al. RNA sequencing analysis reveals protective role of kruppel-like factor 3 in colorectal cancer. Oncotarget 8:21984-21993 (2017). IHC-P ; Human . Read more (PubMed: 28423541) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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