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Rabbit polyclonal to KLF3
Predicted to work with:
Mouse, Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 153-334 of Human KLF3 (UniProt P57682).
A549 and HCT116 cell lysates; SCC4 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Sumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.
Information by UniProt
Basic krueppel-like factor antibody
Basic Kruppel like factor antibody
Western blot - Anti-KLF3 antibody (ab154531)
Anti-KLF3 antibody (ab154531) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 39 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (ab154531)
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft tissue labeling KLF3 with ab154531 at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"