The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/32000 dilution is the detection limit in peptide ELISA.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
Note that the 40kDa Band is sometimes accompanied by a 80 to 90kDa band of unknown identity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Post-translational modificationsSumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.