Overview

  • Product nameAnti-KLF3 antibody
    See all KLF3 primary antibodies
  • Description
    Rabbit polyclonal to KLF3
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: IYSTPLPEKF FQTPEGLSHG IQMEPVDLTV NKRSSPPSAG NSPSSLKFPS, corresponding to N terminal amino acids 37-86 of Human KLF3

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23091163
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

Anti-KLF3 antibody images

  • Anti-KLF3 antibody (ab49221) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-KLF3 antibody (ab49221)

This product has been referenced in:
  • Seifert M  et al. Cellular origin and pathophysiology of chronic lymphocytic leukemia. J Exp Med 209:2183-98 (2012). ICC/IF ; Human . Read more (PubMed: 23091163) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"