Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF3
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: IYSTPLPEKF FQTPEGLSHG IQMEPVDLTV NKRSSPPSAG NSPSSLKFPS, corresponding to N terminal amino acids 37-86 of Human KLF3

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23091163
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Sumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic krueppel-like factor antibody
    • Basic Kruppel like factor antibody
    • BKLF3 antibody
    • CACCC box binding protein BKLF antibody
    • CACCC-box-binding protein BKLF antibody
    • Klf3 antibody
    • KLF3_HUMAN antibody
    • Krueppel-like factor 3 antibody
    • Kruppel like factor 3 (basic) antibody
    • MGC48279 antibody
    • TEF-2 antibody
    • TEF2 antibody
    • Transcript ch138 antibody
    • Zinc finger protein 741 antibody
    • ZNF741 antibody
    see all

Anti-KLF3 antibody images

  • Anti-KLF3 antibody (ab49221) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-KLF3 antibody (ab49221)

This product has been referenced in:
  • Shao M  et al. Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis). Oncotarget 8:16325-16339 (2017). Read more (PubMed: 28032601) »
  • Seifert M  et al. Cellular origin and pathophysiology of chronic lymphocytic leukemia. J Exp Med 209:2183-98 (2012). ICC/IF ; Human . Read more (PubMed: 23091163) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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