Purification notesThis antibody was purified from culture supernatant of hybridoma culturedin a medium containing bovine IgG depleted fetal bovine serum (approximately 95%). Solution was filtered throgh 0.22 µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Detects a band of approximately 35 kDa.
FunctionBinds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Post-translational modificationsSumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.