Overview

  • Product nameAnti-KLF4 antibody [56CT5.1.6]
    See all KLF4 primary antibodies
  • Description
    Mouse monoclonal [56CT5.1.6] to KLF4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
  • Immunogen

    Recombinant fragment corresponding to Human KLF4 aa 10-479.

Properties

Applications

Our Abpromise guarantee covers the use of ab75486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/2000. Predicted molecular weight: 50 kDa.

Target

  • FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Alternative names
    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Anti-KLF4 antibody [56CT5.1.6] images

  • Anti-KLF4 antibody [56CT5.1.6] (ab75486) at 1/200 dilution + KLF4 recombinant protein

    Predicted band size : 50 kDa
  • Immunoflurescence analysis of ab75486 (5 μg/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

References for Anti-KLF4 antibody [56CT5.1.6] (ab75486)

This product has been referenced in:
  • Lee E  et al. DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia 18:553-66 (2016). Human . Read more (PubMed: 27659015) »
  • Gericota B  et al. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med 3:334-45 (2014). Read more (PubMed: 24443004) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO-K1)
Loading amount 35 µg
Specification CHO-K1
Treatment transfected with mouse KLF4 (no tag)
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 09 2012

According to our records, ab75486 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for contacting Abcam regarding ab75486.


I am sorry that you have been experiencing difficulties with this antibody in WB. I am hoping you can provide some additional information to help me resolve the issues you are having wit...

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Thank you for enquiry and your interest in our products.

Currently, testing our antibodies in EMSA is not part of the standard QC process. Most of our products are characterized regularly in WB, IHC, ICC, ELISA, Flow cytometry, ChIP.
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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cell type)
Specification cell type
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Yunhui Cheng

Verified customer

Submitted Mar 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Jennifer Schnabel

Verified customer

Submitted Sep 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"