Overview

  • Product nameAnti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description
    Rabbit polyclonal to KLF4
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1-50 of human KLF4 (NP_004226)

  • Positive control
    • 293, 3T3 and RAW cell lysates and human colon tissue.

Applications

Our Abpromise guarantee covers the use of ab72543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend blocking with 5% nonfat dry milk in TBST for 30-60 minutes. Incubate the membrane overnight with the primary antibody in 1% milk in TBST.
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 101-7 cells.

This antibody has been tested in an intracellular protocol.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Anti-KLF4 antibody images

  • Lanes 1 - 2 : Anti-KLF4 antibody (ab72543) at 1 µg/ml
    Lanes 3 - 4 : Anti-KLF4 antibody (ab72543) at 2 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate with immunizing peptide
    Lane 3 : 3T3 cell lysate
    Lane 4 : RAW cell lysate


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab72543 at 10µg/ml staining KLF4 in human colon by Immunohistochemistry, Paraffin-embedded tissue.
  • Flow analysis of KLF4 on mouse embryonic stem cells using ab72543 at 1:100 dilution. Purple histogram represents rabbit IgG negative control; green line represents anti-KLF4 antibody (ab72543).

    See Abreview

References for Anti-KLF4 antibody (ab72543)

This product has been referenced in:
  • Abu-Hassan DW  et al. Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma. Stem Cells 33:751-61 (2015). Read more (PubMed: 25377070) »
  • Jia D  et al. ß-Catenin and NF-?B co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Cell Death Differ 22:298-310 (2015). Human . Read more (PubMed: 25257174) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Antigen retrieval step None
Permeabilization Yes - tritonx100
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

Submitted Nov 01 2016

Abreviews
Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (undifferentiated human iPS cell)
Specification undifferentiated human iPS cell
Blocking step 5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2014

I can confirm that this antibody has been tested and characterized for Flow cytometry. Since it is not a monoclonal antibody and its isotype has not been determined, it is not possible to recommend an isotype control. You may wish to use rabbit (host s...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem Cell (D3))
Specification Embryonic Stem Cell (D3)
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
Username

Joe Segal

Verified customer

Submitted Sep 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (D3 (Mouse Embryonic Stem Cells))
Loading amount 200000 cells
Specification D3 (Mouse Embryonic Stem Cells)
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Joe Segal

Verified customer

Submitted Sep 04 2012

Since the antibodies ab19857 and ab72543havenot yetbeen tested inon perfused-fixed tissue (IHC-FoFr),I can offer discounts off a future purchase if you purchasethese antibodiesnow, test in IHC-For, and submit feedback to us in the form of Abreviews. It...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cells: D3 cell line)
Loading amount 400000 cells
Specification embryonic stem cells: D3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kate Hawkins

Verified customer

Submitted Apr 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (embryonic stem cells)
Specification embryonic stem cells
Fixation Paraformaldehyde
Permeabilization Yes - 70% Methanol
Gating Strategy all
Username

Dr. Kate Hawkins

Verified customer

Submitted Apr 25 2012

Currently, testing our antibodies in EMSA is not part of the standard QC process. Most of our products are characterized regularly in WB, IHC, ICC, ELISA, Flow cytometry, ChIP.

Currently, we have 8 antibodies against human KLF4. It may be wor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"