Overview

  • Product nameAnti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description
    Goat polyclonal to KLF4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    AVSDALLPSFST-C

    , corresponding to N terminal amino acids 11-22 of Human KLF4 (NP_004226).

  • Positive control
    • Human duodenal lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Anti-KLF4 antibody images

  • Anti-KLF4 antibody (ab77090) at 0.1 µg/ml + Human duodenum lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50,75-90 kDa (why is the actual band size different from the predicted?)

References for Anti-KLF4 antibody (ab77090)

ab77090 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your participation in Abcam's follow up survey.

I have checked for alternative antibodies that were tested in WB as well as with mouse:

ab106629:http://www.abcam.com/klf4-antibody-ab106629.html

We also have a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"