Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 69-346 of Human KLF5 (Uniprot ID: Q13887).

  • Positive control
    • A431, H1299, HeLa and HepG2 whole cell lysates; Mouse ESC D3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificity
    Expressed only in testis and placenta.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
  • Alternative names
    • Basic transcription element binding protein 2 antibody
    • Basic transcription element-binding protein 2 antibody
    • BTE binding protein 2 antibody
    • BTE-binding protein 2 antibody
    • BTEB 2 antibody
    • BTEB2 antibody
    • CKLF antibody
    • Colon krueppel like factor antibody
    • Colon krueppel-like factor antibody
    • GC box binding protein 2 antibody
    • GC-box-binding protein 2 antibody
    • IKLF antibody
    • Intestinal enriched krueppel like factor antibody
    • Intestinal-enriched krueppel-like factor antibody
    • KLF 5 antibody
    • Klf5 antibody
    • KLF5_HUMAN antibody
    • Krueppel like factor 5 antibody
    • Krueppel-like factor 5 antibody
    • Kruppel like factor 5 intestinal antibody
    • Transcription factor BTEB 2 antibody
    • Transcription factor BTEB2 antibody
    see all

Images

  • All lanes : Anti-KLF5 antibody (ab137676) at 1/5000 dilution

    Lane 1 : Non-transfected 293T whole cell extract
    Lane 2 : Transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 51 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • All lanes : Anti-KLF5 antibody (ab137676) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 51 kDa
  • ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500

  • Anti-KLF5 antibody (ab137676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 51 kDa
    10% SDS PAGE

References

This product has been referenced in:
  • Kanatsu-Shinohara M  et al. Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc Natl Acad Sci U S A 111:8826-31 (2014). WB ; Mouse . Read more (PubMed: 24879440) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Human Cell lysate - whole cell (hESC Cells)
Gel Running Conditions
Reduced Denaturing (4-12% Tris Bis)
Loading amount
55000 cells
Specification
hESC Cells
Blocking step
No blocking step used for 24 hour(s) and 0 minute(s) · Temperature: 4°C
Username

Mr. Timothy Hunt

Verified customer

Submitted Sep 12 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 21 2015

Abreviews
Application
Western blot
Loading amount
500000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Cell lysate - whole cell (pancreatic islets)
Specification
pancreatic islets
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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