Overview

  • Product nameAnti-KLF5 antibody
    See all KLF5 primary antibodies
  • Description
    Rabbit polyclonal to KLF5
  • SpecificitySome of our customers have successfully used ab24331 on mouse, please see PubMed ID: 19720868 and 21600198. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our Scientific Support team for further information.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF5.

    (Peptide available as ab30882.)

  • Positive control
    • This antibody gave a positive signal in Human small intestine tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.6 - 3 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21600198
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionTranscription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificityExpressed only in testis and placenta.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormKLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
  • Alternative names
    • Basic transcription element binding protein 2 antibody
    • Basic transcription element-binding protein 2 antibody
    • BTE binding protein 2 antibody
    • BTE-binding protein 2 antibody
    • BTEB 2 antibody
    • BTEB2 antibody
    • CKLF antibody
    • Colon krueppel like factor antibody
    • Colon krueppel-like factor antibody
    • GC box binding protein 2 antibody
    • GC-box-binding protein 2 antibody
    • IKLF antibody
    • Intestinal enriched krueppel like factor antibody
    • Intestinal-enriched krueppel-like factor antibody
    • KLF 5 antibody
    • Klf5 antibody
    • KLF5_HUMAN antibody
    • Krueppel like factor 5 antibody
    • Krueppel-like factor 5 antibody
    • Kruppel like factor 5 intestinal antibody
    • Transcription factor BTEB 2 antibody
    • Transcription factor BTEB2 antibody
    see all

Anti-KLF5 antibody images

  • Anti-KLF5 antibody (ab24331) at 2 µg/ml + Small Intestine (Human) Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • The image shows nuclear staining of the epithelial cells of the intestinal gland. Tris EDTA pH9 and Citrate pH6 are both suitable antigen retrieval techniques.
  • All lanes : Anti-KLF5 antibody (ab24331) at 1/500 dilution

    Lane 1 : Marker ladder
    Lane 2 : Cell lysate prepared from human Ls174T cells at 40 µg
    Lane 3 : Cell lysate prepared from IEC-6 rat intestinal cells at 40 µg

    Secondary
    Lanes 2 - 3 : HRP conjugated goat polyclonal to rabbit IgG at 1/1500 dilution


    Predicted band size : 50 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-KLF5 antibody (ab24331)

This product has been referenced in:
  • Liu JY  et al. MicroRNA-153 inhibits the proliferation and invasion of human laryngeal squamous cell carcinoma by targeting KLF5. Exp Ther Med 11:2503-2508 (2016). WB ; Human . Read more (PubMed: 27284339) »
  • Gao Y  et al. Beyond proliferation: KLF5 promotes angiogenesis of bladder cancer through directly regulating VEGFA transcription. Oncotarget 6:43791-805 (2015). IHC ; Human . Read more (PubMed: 26544730) »

See all 15 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Permeabilization No
Fixative 4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Permeabilization No
Fixative 4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 01 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Airway epithelial cells)
Specification Airway epithelial cells
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Prof. Marc Chanson

Verified customer

Submitted Sep 07 2012

Thank you for your email.

I am sorry this product did not perform in mouseand for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for ab90839. The item is currently backor...

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Thank you for contacting us.

Yes, I would be happy to send you either a free of charge replacement or a credit. Please let me know what you prefer. Which antibody I should send orwhether a credit note would be best.

I look forward...

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Thank you for your reply.

I'd be happy to replace ab24331. Which of the 2 antibodies (ab51317 and ab13970) would you like me to send?

As for ab62341 (which is not affected by the product information sent out for ab24331), we would ...

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Thank you for contacting us.
Your credit note ID for the refund is xxx.
I am sorry that this antibody does not work in mouse as it had been stated on the datasheet. If payment has already been made on the original order and you wish to receiv...

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Thank you for your email.

I am sorry this product did not perform in mouseand for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab5176.

To check the stat...

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Thank you for your email.
I am sorry this product did not perform in mouse and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab72543.
To check the status of the ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience th...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"