Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF5
  • Specificity
    Some of our customers have successfully used ab24331 on mouse, please see PubMed ID: 19720868 and 21600198. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our Scientific Support team for further information.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF5.

    (Peptide available as ab30882.)

  • Positive control
    • This antibody gave a positive signal in Human small intestine tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.6 - 3 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21600198
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificity
    Expressed only in testis and placenta.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
  • Alternative names
    • Basic transcription element binding protein 2 antibody
    • Basic transcription element-binding protein 2 antibody
    • BTE binding protein 2 antibody
    • BTE-binding protein 2 antibody
    • BTEB 2 antibody
    • BTEB2 antibody
    • CKLF antibody
    • Colon krueppel like factor antibody
    • Colon krueppel-like factor antibody
    • GC box binding protein 2 antibody
    • GC-box-binding protein 2 antibody
    • IKLF antibody
    • Intestinal enriched krueppel like factor antibody
    • Intestinal-enriched krueppel-like factor antibody
    • KLF 5 antibody
    • Klf5 antibody
    • KLF5_HUMAN antibody
    • Krueppel like factor 5 antibody
    • Krueppel-like factor 5 antibody
    • Kruppel like factor 5 intestinal antibody
    • Transcription factor BTEB 2 antibody
    • Transcription factor BTEB2 antibody
    see all

Images

  • Anti-KLF5 antibody (ab24331) at 2 µg/ml + Small Intestine (Human) Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • The image shows nuclear staining of the epithelial cells of the intestinal gland. Tris EDTA pH9 and Citrate pH6 are both suitable antigen retrieval techniques.
  • All lanes : Anti-KLF5 antibody (ab24331) at 1/500 dilution

    Lane 1 : Marker ladder
    Lane 2 : Cell lysate prepared from human Ls174T cells at 40 µg
    Lane 3 : Cell lysate prepared from IEC-6 rat intestinal cells at 40 µg

    Secondary
    Lanes 2 - 3 : HRP conjugated goat polyclonal to rabbit IgG at 1/1500 dilution


    Predicted band size : 50 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Liu JY  et al. MicroRNA-153 inhibits the proliferation and invasion of human laryngeal squamous cell carcinoma by targeting KLF5. Exp Ther Med 11:2503-2508 (2016). WB ; Human . Read more (PubMed: 27284339) »
  • Pattison JM  et al. Transcription Factor KLF5 Binds a Cyclin E1 Polymorphic Intronic Enhancer to Confer Increased Bladder Cancer Risk. Mol Cancer Res 14:1078-1086 (2016). Read more (PubMed: 27514407) »

See all 21 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human ESC)
Permeabilization
Yes - 0.5% Triton X in PBS
Specification
human ESC
Blocking step
10% Donkey serum in PBS + 0.1% Tween 20 for 30 minutes at room temperature as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 14 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Permeabilization
No
Fixative
4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 01 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Airway epithelial cells)
Specification
Airway epithelial cells
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Prof. Marc Chanson

Verified customer

Submitted Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (IEC-6 rat intestinal cell line)
Loading amount
40 µg
Specification
IEC-6 rat intestinal cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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