Overview

  • Product nameAnti-KLF5 antibody
    See all KLF5 primary antibodies
  • Description
    Rabbit polyclonal to KLF5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human KLF5.

  • Positive control
    • CEM cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 51 kDa.
ELISA 1/1000.

Target

  • FunctionTranscription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificityExpressed only in testis and placenta.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormKLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
  • Alternative names
    • Basic transcription element binding protein 2 antibody
    • Basic transcription element-binding protein 2 antibody
    • BTE binding protein 2 antibody
    • BTE-binding protein 2 antibody
    • BTEB 2 antibody
    • BTEB2 antibody
    • CKLF antibody
    • Colon krueppel like factor antibody
    • Colon krueppel-like factor antibody
    • GC box binding protein 2 antibody
    • GC-box-binding protein 2 antibody
    • IKLF antibody
    • Intestinal enriched krueppel like factor antibody
    • Intestinal-enriched krueppel-like factor antibody
    • KLF 5 antibody
    • Klf5 antibody
    • KLF5_HUMAN antibody
    • Krueppel like factor 5 antibody
    • Krueppel-like factor 5 antibody
    • Kruppel like factor 5 intestinal antibody
    • Transcription factor BTEB 2 antibody
    • Transcription factor BTEB2 antibody
    see all

Anti-KLF5 antibody images

  • Anti-KLF5 antibody (ab90983) at 1/50 dilution + CEM cell lysate at 35 µg

    Predicted band size : 51 kDa

References for Anti-KLF5 antibody (ab90983)

ab90983 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (VSMC(movas cell line), Macrophage(RAW 264.7))
Loading amount 30 µg
Specification VSMC(movas cell line), Macrophage(RAW 264.7)
Treatment 7-betahydoxy-cholesterol 10-30uM 24hrs , LPS 1ug/ml for 1hrs
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"