Overview

  • Product name
  • Description
    Mouse polyclonal to KLF8
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chinese hamster
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-359 of Human KLF8 (NP_009181.2).

  • Positive control
    • KLF8 transfected 293T cell line lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab168527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic krueppel-like factor 3 antibody
    • BKLF3 antibody
    • DXS741 antibody
    • KLF8 antibody
    • KLF8_HUMAN antibody
    • Krueppel-like factor 8 antibody
    • Kruppel like factor 8 antibody
    • MGC138314 antibody
    • Zinc finger protein 741 antibody
    • ZNF741 antibody
    see all

Images

  • All lanes : Anti-KLF8 antibody (ab168527) at 1 µg/ml

    Lane 1 : KLF8 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Predicted band size: 39 kDa

  • Immunofluorescent analysis of HeLa cells labeling KLF8 with ab168527 at 10 µg/ml.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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