- Datasheet
- Specific References
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Overview
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Product nameAnti-KLF9 antibody
See all KLF9 primary antibodies -
DescriptionRabbit polyclonal to KLF9
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
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Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 28-57 of Human KLF9 (NP_001197.1).
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Positive control
- WiDr cell line lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab124145 was purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab124145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 27 kDa. | |
| ICC/IF | 1/10 - 1/50. |
Target
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FunctionTranscription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box.
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Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 687 Human
- Entrez Gene: 16601 Mouse
- Entrez Gene: 397370 Pig
- Entrez Gene: 117560 Rat
- Omim: 602902 Human
- SwissProt: Q13886 Human
- SwissProt: O35739 Mouse
- SwissProt: P79288 Pig
see all -
Alternative names
- Basic transcription element binding protein 1 antibody
- Basic transcription element-binding protein 1 antibody
- BTE binding protein 1 antibody
see all
Anti-KLF9 antibody images
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Immunofluorescence - Anti-KLF9 antibody (ab124145)Immunofluorescence of U251 cells labelling KLF9 with ab124145. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab124145 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and the nucleus.
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Western blot - Anti-KLF9 antibody (ab124145)Anti-KLF9 antibody (ab124145) at 1/100 dilution + WiDr cell line lysate at 35 µg
Predicted band size : 27 kDa
References for Anti-KLF9 antibody (ab124145)
ab124145 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"