Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF9
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 28-57 of Human KLF9 (NP_001197.1).

  • Positive control
    • WiDr cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab124145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 27 kDa.
ICC/IF 1/10 - 1/50.

Target

Images

  • Immunofluorescence of U251 cells labelling KLF9 with ab124145. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab124145 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and the nucleus.

  • Anti-KLF9 antibody (ab124145) at 1/100 dilution + WiDr cell line lysate at 35 µg

    Predicted band size : 27 kDa

References

ab124145 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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