Anti-KLF9 antibody (ab227920)
Key features and details
- Rabbit polyclonal to KLF9
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLF9 antibody
See all KLF9 primary antibodies -
Description
Rabbit polyclonal to KLF9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human KLF9 aa 1 to the C-terminus.
Database link: Q13886 -
Positive control
- IP: HeLa whole cell extract. WB: HeLa whole cell and nuclear extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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Notes |
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IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
Target
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Function
Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539139 Cow
- Entrez Gene: 687 Human
- Entrez Gene: 16601 Mouse
- Entrez Gene: 397370 Pig
- Entrez Gene: 117560 Rat
- Omim: 602902 Human
- SwissProt: Q13886 Human
- SwissProt: O35739 Mouse
see all -
Alternative names
- Basic transcription element binding protein 1 antibody
- Basic transcription element-binding protein 1 antibody
- BTE binding protein 1 antibody
see all
Images
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All lanes : Anti-KLF9 antibody (ab227920) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa12% SDS-PAGE gel.
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KLF9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab227920. Western blot analysis was performed using ab227920. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Control IgG instead of ab227920 in HeLa whole cell extract.
Lane 2: ab227920 IP in HeLa whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab227920 has been referenced in 12 publications.
- Liu Y et al. KLF9 regulates miR-338-3p/NRCAM axis to block the progression of osteosarcoma cells. J Cancer 13:2029-2039 (2022). PubMed: 35399713
- Li T et al. Overexpression of miRNA-93-5p Promotes Proliferation and Migration of Bladder Urothelial Carcinoma via Inhibition of KLF9. Comput Math Methods Med 2022:8911343 (2022). PubMed: 35495878
- Chen Q et al. Circular RNA_0078767 upregulates Kruppel-like factor 9 expression by targeting microRNA-889, thereby inhibiting the progression of osteosarcoma. Bioengineered 13:14313-14328 (2022). PubMed: 35758280
- Ong JR et al. SUMO-Activating Enzyme Subunit 1 (SAE1) Is a Promising Diagnostic Cancer Metabolism Biomarker of Hepatocellular Carcinoma. Cells 10:N/A (2021). PubMed: 33477333
- Ai F et al. Dexamethasone induces aberrant macrophage immune function and apoptosis. Oncol Rep 43:427-436 (2020). PubMed: 31894280